Journal: Oncology Letters

Article Title: Chemopreventive effect of 4′-hydroxychalcone on intestinal tumorigenesis in Apc Min mice

doi: 10.3892/ol.2021.12474

Figure Lengend Snippet: Oral administration of 4-HC prevents spontaneous intestinal polyposis in ApcMin mice. (A) Representative images of colon polyps in ApcMin mice treated with 4-HC or Veh for 12 weeks. Images were depicted macroscopically (scale bar, 10 mm; top images) and under a stereoscopic dissection microscope (scale bar, 1 mm; bottom images). ApcMin mice treated with 10 mg/kg/day 4-HC (n=12) and Veh (n=11) for 12 weeks and (B) colon polyp number, (C) DSI/PSI polyp number, (D) colon polyp size, and (E) DSI/PSI polyp size were analyzed. Polyp size is the tumor surface area observed under the microscope. (F) Representative images of polyps in the small intestine of ApcMin mice under a stereoscopic dissection microscope. Scale bar, 1 mm. Representative images of adenoma burden in the (G) colon and (H) DSI from mice treated with Veh or 4-HC. Green triangles indicate microscopic intestinal adenomas. Scale bar, 2 mm. Data are presented as the mean ± SEM. *P<0.05, **P<0.01. 4-HC, 4′-hydroxychalcone; ApcMin , adenomatous polyposis coli multiple intestinal neoplasia mouse model; DSI, distal small intestine; ns, not significant; PSI, proximal small intestine; Veh, vehicle.

Article Snippet: A stereoscopic dissection microscope (Stemi 2000-c; Carl Zeiss AG) was utilized to assess the tumor burden by counting the number of adenomas and determining their dimensions (AxioVision Application, version 4.6, Cal Zeiss Microscopy).

Techniques: Dissection, Microscopy

Journal: PLOS ONE

Article Title: Topic: Distribution of Anopheles stephensi bioforms in selected districts of Rajasthan, India

doi: 10.1371/journal.pone.0313227

Figure Lengend Snippet: Wild gravid females were allowed to lay eggs, and subsequently, the egg ridges were counted and measured using an Occulo-meter. In this figure, 19 ridges were observed and counted under the microscope which is a ‘Type’ bioform.

Article Snippet: For morphometric analysis, the length, breadth, and number of ridges on the egg float were measured under a compound microscope (Zeiss stem 2000-c) at 10x and 40x magnification using an occulo-meter.

Techniques: Microscopy